No vaccines exist that protect people against
infections by coronaviruses, including SARS-CoV-2, which causes
COVID-19, or the ones that cause SARS and MERS. As COVID-19 continues to
wreak havoc, many labs around the world have developed a laser-like
focus on understanding the virus and finding the best strategy for
stopping it. Researchers now suggest that the approach they took for a
MERS virus vaccine may also work against SARS-CoV-2.}=> No vaccines exist that protect people against infections by
coronaviruses, including SARS-CoV-2, which causes COVID-19, or the ones
that cause SARS and MERS. As COVID-19 continues to wreak havoc, many
labs around the world have developed a laser-like focus on understanding
the virus and finding the best strategy for stopping it.
=>This week in mBio, a journal of the American Society of
Microbiology, a team of interdisciplinary researchers describes a
promising vaccine candidate against the MERS virus. Since the MERS
(Middle East Respiratory Syndrome) outbreak began in 2012, more than 850
people have died, and studies suggest the virus has a case fatality
rate of more than 30%.
In the new paper, the researchers suggest that the approach they took
for a MERS virus vaccine may also work against SARS-CoV-2. The
vaccine's delivery method is an RNA virus called parainfluenza virus 5
(PIV5), which is believed to cause a condition known as kennel cough in
dogs but appears harmless to people. The researchers added an extra gene
to the virus so that infected cells would produce the S, or spike,
glycoprotein known to be involved in MERS infections.
=>"We know people have been exposed to PIV5, but it seems to be an
innocuous virus in humans," said pediatric pulmonologist and coronavirus
expert Paul McCray, M.D., at the University of Iowa, in Iowa City, who
co-led the new study with virologist Biao He, Ph.D., at the University
of Georgia, in Athens. "PIV5 doesn't seem to cause a cytopathic effect."
The MERS virus cannot replicate in mice, so to test the vaccine McCray
developed a mouse model that mimics human infections. The mice had been
genetically engineered to express DPP4, the protein used by the MERS
virus as an entry point for human cells.
Lab tests showed that a single dose of the vaccine, given
intranasally, effectively caused infected cells to produce the S
protein, which in turn triggered immune responses against the protein in
the animal host.
Four weeks after the mice received the vaccine, they were exposed to a
strain of the MERS virus, adapted to the mice to cause a lethal
infection. The MERS virus was also given to groups of mice that had
received a different PIV5 vaccine -- one without the genes for the S
protein -- or an intramuscular vaccine with inactivated MERS virus.
All the mice immunized with the modified PIV5 virus survived MERS
virus infection. In contrast, all the mice immunized with the PIV5
without S died from the infection. The intramuscular vaccine of
inactivated MERS virus only protected 25% of the mice from a lethal
infection. The mice that received inactivated MERS virus showed
above-average levels of eosinophils, white blood cells that indicate
infection or inflammation. This connection raises a safety concern for
inactivated MERS virus as a potential vaccine, said He. The study
demonstrates that an intranasal, PIV5-based vaccine is effective against
MERS in mice, said He, and should be investigated for its potential
against other dangerous coronaviruses, including SARS-CoV-2.
=>"We're quite interested in using viruses as gene delivery vehicles,"
said McCray, who has also investigated similar strategies as a way to
treat cystic fibrosis. Now, like colleagues around the world, McCray and
He have both focused their research efforts on SARS-CoV-2, taking a
similar tack to working with mouse models of infection and testing
vaccines.
Finding an effective vaccine against the coronavirus that causes
COVID-19 is a race against time, McCray said. "One hundred percent of
the population is not going to be exposed to the virus the first time
around, which means there will be more people to infect when it comes
again," he said. "We don't know yet if people get lasting immunity from
the SARS-CoV-2 infection, so it's important to think about ways to
protect the population."*****
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